Research Seminar of Dr. Andrei V. Budanovfrom Virginia Commonwealth University

Lecture Time: 10:00 on 2nd March, 2016

Lecture Room: C501 of National Sicence Park

ANDREI V. BUDANOV
VIRGINIA COMMONWEALTH UNIVERSITY
DEPARTMENT OF HUMAN AND MOLECULAR GENETICS
MASSEY CANCER CENTER, GOODWIN BLDG., RM288
401 COLLEGE STREET, RICHMOND VA 23298
CELL: 1-858-822-8945 E-MAIL:ABUDANOV@VCU.EDU
CITIZENSHIP: USA/RUSSIA

EDUCATION
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2002 ENGELHARDT INSTITUTE OF MOLECULAR BIOLOGY, RUSSIAN ACADEMY OF SCIENCES, Moscow, Russia
Ph.D. in Molecular Biology
Dissertation advisor: Dr. Peter M. Chumakov
Dissertation title: "Identification and characterization of a novel stress-responsive gene Hi95/Sestrin2"

1996 LOMONOSOV MOSCOW STATE UNIVERSITY, Moscow, Russia
BS/MS in Biochemistry and Virology

EMPLOYMENT
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Dates Organization Department Supervisor Position
2013-now Virginia Commonwealth University Human and Molecular Genetics Assistant Professor
2011-2013 Virginia Commonwealth University Neurosurgery/Biochemistry Assistant Professor
2008-2011 University of California San Diego Pharmacology prof. Michael Karin Project Scientist
2004-2008 University of California San Diego Pharmacology prof. Michael Karin Postdoctoral Fellow
2002-2004 The Cleveland Clinic Foundation Molecular Genetics prof. Peter Chumakov Research Fellow
2000-2001 University of Illinois at Chicago Molecular Biology prof. Peter Chumakov Visiting Scholar
1999 University of Illinois at Chicago Molecular Biology prof. Andrei Gudkov Visiting Scholar

AWARDS AND FELLOWSHIPS
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2013-2018 NIH RO1 "Role of p53-inducible Sesn1 and Sesn2 genes in lung carcinogenesis". CA172660 Budanov PI
2008-2013 NIH K99/R00 "Role of p53 and p53-inducible Sestrin1 and 2 genes in hepatocarcinogenesis". DK082080 Budanov PI
2005-2007 Tobacco Related Diseases Research Program, University of California, Postdoctoral fellowship "Role of p53-regulated Sestrin genes in lung cancer".
2004 The Cleveland Clinic Foundation William E. Lower Fellowship Thesis Prize for the Best Publication
2004 Oxygen Club California BASF, Young Investigator Award
2001 FEBS Forum of Young Scientists; Protein Structure-Function Trafficking and Signaling; 27th FEBS meeting, travel award
2000 FEBS-EMBO International Summer School Spetses; Molecular Mechanisms of Development and Disease, travel award
1999 International Union Against Cancer (UICC), Research Fellowship

PUBLICATIONS
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1. Parmigiani A, and Budanov AV. (2016) Sensing the environment through Sestrins: implications for cellular metabolism. (Review). International Review of Cell and Molecular Biology (in press).

2. Ding B, Parmigiani A, Divakaruni AS, Murphy AN, and Budanov AV. (2016) Sestrin2 is induced by glucose starvation via the unfolded protein response and protects cells from non-canonical necroptotic cell death. Scientific Reports (in press).

3. Tsilioni I, Filippidis A, Kerenidi T, Budanov AV, Zarogiannis SG, and Gourgoulianis KI. (2016) Sestrin-2 is significantly increased in malignant pleural effusions due to lung cancer and is potentially secreted by pleural mesothelial cells. Clinical Biochemistry Feb 11 epub ahead of print.

4. Ding B, Parmigiani A, Yang C and Budanov AV. Sesn2 controls cell death in response to proinflammatory cytokines in lung adenocarcinoma cells. Cell Cycle 14(20):3231-41

5. Budanov AV. (2015) SESTRINS regulate mTORC1 via RRAGs: The riddle of GATOR. Molecular and Cellular Oncology 2(3): e997113-1-3

6. Parmigiani A, Nourbakhsh A, Ding B, Wang W, Kim YC, Akopiants K, Guan KL, Karin M, and Budanov AV. (2014) Sestrins inhibit mTORC1 kinase activation through the GATOR complex. Cell Reports 9(4): 1281-91 PMID: 25457612 (cited 32 times)

7. Morrison A, Chen L, Wang J, Zhang M, Yang H, Ma Y, Budanov A, Lee JH, Karin M, and Li J. (2014).Sestrin2 promotes LKB1-mediated AMPK activation in the ischemic heart. FASEBJ 29(2):408-17 PMID: 25366347 (cited 4 times)

8. Zhao B, Shah P, Budanov A, Qiang L, Ming M, Aplin A, Sims DM, and He YY. (2014). Sestrin2 sitively regulates AKT signaling and survival in human squamous cell carcinoma and melanoma cells. Journal of Biological Chemistry 289(52):35806-14 PMID: 25378405 (cited 2 times)

9. Lee JH*, Budanov AV* [*equal contribution], and Karin M. (2013). Sestrins orchestrate cellular metabolism to attenuate aging. (Review). Cell Metabolism 18(6): 792-801. PMID: 24055102 (cited 26 times)

10. Ben-Sahra I, Dirat B, Lauren K, Puissant A, Auberger P, Budanov A, Tanti JF, and Bost F. (2012). Sestrin2 integrates Akt and mTOR signaling to protect cells against energetic stress-induced death. Cell Death and Differentiation 20(4):611-619. PMID: 23238567 (cited 28 times)

11. Lee JH*, Budanov AV* [*equal contribution], Talukdar S, Park EJ, Park HL, Park HV, Bandyopadhyay G, Li N, Aghajan M, Jang I, Wolfe AM, Perkins GA, Ellisman MH, Bier E, Scadeng M, Foretz M, Violet B, Olefsky J, and Karin M. (2012). Maintenance of metabolic homeostasis by sestrin2 and sestrin3. Cell Metabolism 16(3):311-321. PMID: 22958918 (cited 45 times)

12. Budanov AV (2010). Stress-responsive Sestrins link p53 with redox regulation and mTOR signaling. (Review). Antioxidant & Redox Signaling 15:1679-1690. PMID: 20712410 (cited 37 times)

13. Budanov AV, Lee JH, and Karin M. (2010). Stressin’ Sestrins take an aging fight. (Review). EMBO Molecular Medicine 2: 388-400. PMID: 20878915 (cited 46 times)

14. Lee JH, Budanov AV, Park EJ, Kim TE, Perkins GA, Ocorr K, Ellisman MH, Bodmer E, Bier E, and Karin M (2009). Sestrin is a feedback inhibitor of mTOR that prevents age-related pathologies. Science 327: 1223-1228. PMID:20203043 (cited 161 times).

15. Budanov AV and Karin M. (2008). The p53-responsive Sestrin gene products inhibit mTOR signaling. Cell 134: 451-460. PMID: 18692468 (cited 432 times).
16. Sablina AA*, Budanov AV* [equal contribution], Ilyinskaya GV, Agapova LS, Kravchenko JE, and Chumakov PM. (2005). The antioxidant function of the p53 tumor suppressor. Nature Medicine 11(12):1306-1313. PMID: 16286925 (cited 535 times).

17. Budanov AV, Sablina AA, Feinstein E, Koonin E, and Chumakov PM. (2004). Regeneration of Peroxiredoxins by p53-Regulated Sestrins, Homologs of Bacterial AhpD. Science 304:596-600. PMID: 15105503 (cited 393 times).

18. Budanov AV, Shoshani T, Faerman A, Zelin E, Kamer I, Kalinski H, Gorodin S, Fishman A, Chajut A, Einat P, Skaliter R, Gudkov AV, Chumakov PM, and Feinstein E. (2002). Identification of a novel stress responsive gene Hi95 involved in regulation of cell viability. Oncogene 21: 6017-6031. PMID: 12203114 (cited 113 times).

19. Kochetkov DV, Ilyinskaya GV, Komarov PG, Strom E, Agapova LC, Ivanov AV, Budanov AV, Frolova EI, and Chumakov PM. (2007). Transcriptional inhibition of human papiloma virus in cervical carcinoma cells reactivates functions of the tumor suppressor p53. Molecular Biology (Moscow) 41(3):515-523. PMID: 17685229 (cited 11 times).

20. Razorenova OV, Agapova LS, Budanov AV, Ivanov AV, Strunina SM, and Chumakov PM. (2005). Retroviral reporter systems for the assessment of activity of stress-induced signal transduction pathways controlled by p53, HIF-1 and HSF-1 transcription factors. Molecular Biology (Moscow) 39(2): 286-93. PMID: 15856952 (cited 12 times).

21. Razorenova OV, Ivanov AV, Budanov AV, and Chumakov PM. (2005). Virus-based reporter systems for monitoring transcriptional activity of hypoxia-inducible factor 1. Gene 350(1):89-98. PMID: 15794924 (cited 21 times).

22. Gurova KV, Rokhlin OW, Budanov AV, Burdelya LG, Chumakov PM, Coen MB, and Gudkov AV. (2003). Cooperation of Two Mutant p53 Allels Contributes to Fas Resistance of Prostate Carcinoma Cells. Cancer Research 63:2905-2912. PMID: 12782597 (cited 39 times).

23. Shoshani T, Faerman A, Mett I, Zelin E, Tena T, Gorodin S, Moshel Y, Elbaz S, Budanov A, Chajut A, Kalinski H, Kamer I, Rozen A, Mor O, Keshet E, Leshkovitz D, Einat P, Skaliter R, and Feinstein E. (2002). Identification of a Novel Hipoxia-Inducible Factor 1-responsible Gene RTP801, involved in Apoptosis. Molecular and Cellular Biology 7:2283-2293. PMID: 11884613 (cited 309 times).

24. Smirnov AS, Ruzov AS, Budanov AV, Prokhortchouk AV, Ivanov AV, and Prokhortchouk EB. (2001). High constitutive level of NF-kappaB is crucial for viability of adenocarcinoma cells. Cell Death and Differentiation 8:621-630. PMID: 11536013 (cited 17 times).

25. Smirnov AS, Budanov AV, Ruzov AS, Ivanov AV, Prokhortchouk AV, Gnuchev NV, Prokhortchouk EB. (2000). A high constitutive level of NF-kappa B is necessary for viability of murine adenocarcinoma cells-possible role of p53. Molecular Biology (Moscow) 34(5):775-82. PMID:11033801 (cited 1 time)

26. Neverova ME, Budanov AV, Adamskaya EI, Prasolov VS, and Kalinin ME. (2000). Construction by using retrovirus vector of a recombinant with synthetic bradykinin gene for investigations of human gene expression in mammalian cells. Vest. Ross. Akad. Med. Nauk (Russia). 7:33-35. PMID: 10961146 (cited 2 times)

BOOK CHAPTERS
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Budanov AV. The role of tumor suppressor p53 in the antioxidant defense and metabolism. Chapter in the book "p53 mutations and MDM2 overexpression and cancer". (2014). Subcellular Biochemistry, Springer, 85:337-358. PMID: 25201203

Budanov AV. Sestrins Link Tumor Suppressors with the AMPK-TOR signaling network. (2012) Chapter in the book "Protein Phosphorylation in Human Health." InTech

PRESENTATIONS
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2015 Seminar talk, Wake Forest School of Medicine, Winston-Salem, NC
2014 Seminar talk, Skolkovo Institute of Technology, Skolkovo, Russia
2014 Seminar talk, Netherlands Cancer Institute (NKI), Amsterdam, Netherlands
2013 Seminar talk, Department of Biochemistry, School of Medicine, West Virginia University, Morgantown WV, USA
2013 Seminar talk, Center for Gene Regulation in Health and Diseases, Cleveland State University, Cleveland OH, USA
2013 Seminar talk, Department of Pharmacological Sciences, School of Medicine, Stony Brook University
2013 Invited speaker, V. Else Kr?ner-Fresenius Symposium on Adult Stem Cells in Aging, Diseases, and Cancer, Eisenach, Germany
2011 Seminar talk, Ulm University, Ulm, Germany
2011 Seminar talk, Department of Biochemistry, National University of Singapore, Singapore
2011 Seminar talk, College of Science, Koc University, Istamnul, Turkey
2009 Research talk, Edinburgh Cancer Research Center, Edinburgh, UK
2008 Research talk; 14th International p53 Workshop; Shanghai, China
2008 Cancer and Cell Biology Seminar Series Presentation, University of Cincinnati, Cincinnati OH, USA
2004 Research talk; Superfund Basic Research Program Seminar; University California San Diego, La Jolla CA, USA
2004 Research talk Symposium: p53 and Beyond; School of Bioengineering and Bioinformatics, Moscow State University, Moscow, Russia

TEACHING EXPERIENCE
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1996-2013 Supervised undergraduate and graduate students in the Engelhardt Institute of Molecular Biology, The Cleveland Clinic, University California San Diego and Virginia Commonwealth University.
2013-now Lecture course for graduate and medical students at VCU: "Cancer".
2013-now Lecture course for graduate students at VCU: "Cell Signaling"

MEETINGS ATTENDED
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2015 AACR Meeting Integrating Metabolism and Tumor Biology, Vancouver, Canada
2014 AACR Meeting Molecular Origins of Lung Cancer, San Diego, CA
2013 V. Else Kr?ner-Fresenius Symposium on Adult Stem Cells in Aging, Diseases, and Cancer,
Eisenach, Germany
2013 AACR Annual Meeting 2013
2010 Microscopy: from genome scale to the single molecule, EMBO World Practical Course, Pretoria,
South Africa
2009 DNA microarray - analysis and applications, EMBO World Practical Courses, Tamil Nadu, India
2009 Cancer Proteomics 2009: mechanistic insights, technological advances and molecular medicine, Dublin, Ireland
2008 14th International p53 Workshop; Shanghai, China
2008 Keystone Symposia "Cancer and Microenvironment" (conference assistant)
2007 HHMI sponsored Lab Management Course; San Diego, CA
2004 12 th International p53 Workshop; Dunedin, New Zealand
2004 Cleveland Biology Symposium "Regulation of Nuclear Functions"; Cleveland, OH
2004 11th Annual meeting of the Oxygen Club of California, Santa Barbara, CA
2002 11th International p53 Workshop; Barcelona, Spain
2001 27th Meeting of the Federation European Biochemical Societies, Lisbon, Portugal

MEMBERSHIPS
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American Association for Cancer Research (AACR)
Russian Biochemical Society

PATENTS
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61/358,281 Michael Karin, Jun Hee Lee, and Andrei Budanov 2010
SESTRINS AND SESTRIN ANALOGS AS ANTI-AGING DRUGS AND AS THERAPIES FOR DEGENERATIVE DISEASES

REVIEWER
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Ad hoc reviewer of grants for German Research Foundation (DFG), National Institute of Health Veteran Affair (USA), and VCU Massey Cancer Center pilot projects