李艳,博士,研究员,硕士生导师。2008年于新利18彩票 获生物化工专业工学博士学位。2006—2009年先后三次在德国斯图加特大学生物化学技术研究所和生物工程研究所作为客座学者开展项目合作。2008年至今在新利18彩票 生物与制药工程学院从事教学科研工作。研究领域涉及酶工程、天然产物生物催化转化及分离等。近年来主要在糖酶的开发与应用,以及甜菊糖苷系列产品、AA2G、槲皮素糖苷等糖基化学品的生物合成方面积累了一定经验。目前在研课题包括国家自然科学基金面上项目和企业委托项目。已发表SCI/EI及国内核心期刊论文20余篇,申请国家发明专利10余项,其中授权6项。
代表性论文
1.Chen L, Cai R, Weng J,Li Y*, Jia H, Chen K, Yan M, Ouyang P. Production of rebaudioside D from stevioside using a UGTSL2 Asn358Phe mutant in a multi-enzyme system. Microbial Biotechnology. 2020. doi:10.1111/1751-7915.13539
2.Sun P, Cai R, Chen L,Li Y*, Jia H, Yan M, Chen K. Natural product glycosylation: Biocatalytic synthesis of quercetin-3, 4′-O-diglucoside. Applied Biochemistry and Biotechnology, 2020, 190: 464–474
3.Li Y*, Li Z, He X, Chen L, Cheng Y, Jia H*, Yan M, Chen K. Characterisation of aThermobacillussucrose phosphorylase and its utility in enzymatic synthesis of 2-O-alpha-D-glucopyranosyl-L-ascorbic acid. Journal of Biotechnology, 2019, 305: 27–34
4.Chen L, Sun P, Zhou F,Li Y*, Chen K, Jia H, Yan M, Gong D, Ouyang P. Synthesis of rebaudioside D, using glycosyltransferase UGTSL2 andin situUDP-glucose regeneration. Food Chemistry, 2018, 259: 286-291
5.Cai R, Chen C,Li Y*, Sun K, Zhou F, Chen K, Jia H. Improved soluble bacterial expression and properties of the recombinant flavonoid glucosyltransferase UGT73G1 fromAllium cepa. Journal of Biotechnology, 2017, 255: 9-15
6.Chen L, Sun P,Li Y*, Yan M, Xu L, Chen K, Ouyang P. A fusion protein strategy for soluble expression ofSteviaglycosyltransferase UGT76G1 inEscherichia coli. 3 Biotech, 2017, 7(6):356
7.Li Y*, Li Y, Wang Y, Chen L, Yan M, Chen K, Xu L, Ouyang P. Production of rebaudioside A from stevioside catalyzed by the engineeredSaccharomyces cerevisiae. Applied Biochemistry & Biotechnology, 2016, 178(8): 1586-1598
8.Wang Y, Chen L,Li Y*,Li Y, Yan M, Chen K, Hao N, Xu L. Efficient enzymatic production of rebaudioside A from stevioside. Bioscience Biotechnology & Biochemistry, 2015, 80(1): 67-73